t vector meaning in English
t向量
Examples
- Then the pcr product was purified , ligated into pgem - t vector by ta cloning
筛选阳性克隆，测序，大量制备序列完全正确的质粒。 - The pcr product was cloned into pmd18 - t vector . the positive recombinant clone was identified by pcr and endonuclease digest
提取重组质粒经pcr鉴定和酶切鉴定后，对插入片段进行序列测定及分析。 - The product of pcr named vp6 is approximate 1 . 3kb in length . the vp6 gene was cloned into pmd18 - t vector and sequenced
将其插入克隆载体质粒pmd18 - t的ecorv酶切位点处，构建重组质粒pmd18 - t ? vp6 。 - Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
Pgem一t载体，感受态细菌jm109 ，反转录试剂盒和体外翻译试剂盒均购自promega公司。 - 2 . pcr products can be purified by ctab method , which removing the dntp and primers . the purified products can be used in the ligation reaction to pmd18 - t vector with high efficiency
2 、采用ctab进行pcr产物的纯化，以去除pcr产物中的引物和dntp ，所得产物可用于与t载体连接反应，以提高连接效率。